Milford Molecular Diagnostics

2044 Bridgeport Ave, Milford, CT 06460


Lyme Disease DNA Test

"DNA sequencing-based diagnostics are our specialty and routine diagnostic DNA sequencing is the gateway to practicing personalized molecular medicine."

Sin Hang Lee, MD, F.R.C.P.(C), FCAP


Comprehensive Lyme disease DNA Testing


Clinical Lyme disease and related borrelioses are a group of infectious diseases which may be caused by Borrelia burgdorferi sensu lato including B. mayonii [1], B. miyamotoi [2], B. hermsii, B. turicatae, B. parkerii [3], and other unnamed borrelia strains [4,5]. 

Following the well-established method to compare and identify bacteria, as promulgated by the CDC [6], we are using a set of broad spectrum genus-specific primers to perform nested PCR amplification of a conserved segment of the 16S rRNA genes of the borrelia species listed in Table 1 for DNA sequencing-based molecular diagnosis.

Table 1 (modified from Lee SH et al. Int J Mol Sci. 2014; 15:11364-11386.)

Data retrieved from the National Center for Biotechnology Information (GenBank)


Our method can detect all of the above plus novel strains of borrelia [5]. All tested samples are issued an official laboratory report with an accompanying DNA sequencing electropherogram and GenBank BLAST alignment analysis if the result is positive.  




[1] Pritt BS et al. Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study. Lancet Infect Dis. 2016 Feb 5. pii: S1473-3099(15)00464-8. doi: 10.1016/S1473-3099(15)00464-8.

[2] Gugliotta JL, et al. Meningoencephalitis from Borrelia miyamotoi in an immunocompromised patient. N Engl J Med. 2013;368:240–245.

[3] CDC. Tick-borne relapsing fever.

[4] Lee SH, et al. Detection of Borreliae in Archived Sera from Patients with Clinically Suspect Lyme Disease. Int J Mol Sci. 2014; 15:4284-4298.

[5]Lee SH. Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report. Int Med Case Rep J. 2016; 9:101–106.

[6] CDC. MicrobeNet.