Milford Molecular Diagnostics

2044 Bridgeport Ave, Milford, CT 06460

 


Lyme Disease DNA Test


"DNA sequencing-based diagnostics are our specialty and routine diagnostic DNA sequencing is the gateway to practicing personalized molecular medicine."


Sin Hang Lee, MD, F.R.C.P.(C), FCAP



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Comprehensive Lyme disease DNA Testing

 

Clinical Lyme disease and related borrelioses are a group of infectious diseases which may be caused by Borrelia burgdorferi sensu lato including B. mayonii [1], B. miyamotoi [2], B. hermsii, B. turicatae, B. parkerii [3], and other unnamed borrelia strains [4,5]. 


Following the well-established method to compare and identify bacteria, as promulgated by the CDC [6], we are using a set of broad spectrum genus-specific primers to perform nested PCR amplification of a conserved segment of the 16S rRNA genes of the borrelia species listed in Table 1 for DNA sequencing-based molecular diagnosis.



Table 1 (modified from Lee SH et al. Int J Mol Sci. 2014; 15:11364-11386.)

Data retrieved from the National Center for Biotechnology Information (GenBank)


 

Our method can detect all of the above plus novel strains of borrelia [5]. All tested samples are issued an official laboratory report with an accompanying DNA sequencing electropherogram and GenBank BLAST alignment analysis if the result is positive.  

 

 



References

[1] Pritt BS et al. Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study. Lancet Infect Dis. 2016 Feb 5. pii: S1473-3099(15)00464-8. doi: 10.1016/S1473-3099(15)00464-8.

[2] Gugliotta JL, et al. Meningoencephalitis from Borrelia miyamotoi in an immunocompromised patient. N Engl J Med. 2013;368:240–245.

[3] CDC. Tick-borne relapsing fever. http://www.cdc.gov/relapsing-fever/clinicians/

[4] Lee SH, et al. Detection of Borreliae in Archived Sera from Patients with Clinically Suspect Lyme Disease. Int J Mol Sci. 2014; 15:4284-4298.

[5]Lee SH. Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report. Int Med Case Rep J. 2016; 9:101–106.

[6] CDC. MicrobeNet.  http://www.cdc.gov/microbenet/about.html