Comprehensive Lyme disease DNA
Lyme disease and related borrelioses are a group of infectious diseases which may be
caused by Borrelia burgdorferi sensu
lato including B. mayonii , B. miyamotoi
, B. hermsii, B. turicatae, B.
parkerii , and other unnamed borrelia strains [4,5].
the well-established method to compare and identify bacteria, as promulgated by
the CDC , we are using a set of broad spectrum genus-specific primers to perform nested PCR
amplification of a conserved segment of the 16S rRNA genes of the borrelia
species listed in Table 1 for DNA sequencing-based molecular diagnosis.
Table 1 (modified from Lee SH et al. Int J Mol Sci. 2014; 15:11364-11386.)
Data retrieved from the National Center for Biotechnology Information (GenBank)
method can detect all of the above plus novel strains of borrelia . All
tested samples are issued an official laboratory report with an
accompanying DNA sequencing electropherogram and GenBank BLAST alignment
analysis if the result is positive.
 Pritt BS et al. Identification of a novel pathogenic
Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia:
a descriptive study. Lancet Infect Dis. 2016 Feb 5. pii: S1473-3099(15)00464-8.
 Gugliotta JL, et al. Meningoencephalitis from
Borrelia miyamotoi in an immunocompromised patient. N Engl J Med.
 CDC. Tick-borne relapsing fever. http://www.cdc.gov/relapsing-fever/clinicians/
 Lee SH, et al. Detection of Borreliae in Archived
Sera from Patients with Clinically Suspect Lyme Disease. Int J Mol Sci. 2014;
Lee SH. Lyme disease caused by Borrelia burgdorferi
with two homeologous 16S rRNA genes: a case report. Int Med Case Rep J. 2016;
 CDC. MicrobeNet.